Cat. #151475
Raf KI D486A Mouse
Cat. #: 151475
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Raf KI D486A Mouse
- Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
- Tool sub type: Mouse
- Disease: Cancer
- Conditional: No
- Description: Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
- Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Raf1 D486A (oncogenic mutant)
References
- Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
- BRAF inactivation drives aneuploidy by deregulating CRAF.
- Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
- CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
