#151467

p23+/- Mouse

Cat. #151467

p23+/- Mouse

Cat. #: 151467

Sub-type: Mouse

Availability: 6-8 weeks

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Mike Owen

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: p23+/- Mouse
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
  • Tool sub type: Mouse
  • Description: In vivo study of p23 knockout, golgi apparatus and early secretory pathway function
  • Genetic background: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
  • Phenotype: Golgi apparatus abnormalities
  • Zygosity: Heterozygous
  • Strain: 129/C57BL/6
  • Production details: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
  • Additional notes: Genetic Bkg: 129/C57BL/6. Zygosity: Heterozygous

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: p23

References

  • Denzel et al. 2000. Curr Biol. 10(1):55-8. PMID: 10660306.
  • The p24 family member p23 is required for early embryonic development.