Cat. #158395
Pik3ca-H1047R conditional Flp inducible
Cat. #: 158395
Sub-type: Mouse
Availability: 6-8 weeks
Model: Conditional KO
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Contributor
Inventor: Bart Vanhaesebroeck
Institute: Ludwig Institute for Cancer Research
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Pik3ca-H1047R conditional Flp inducible
- Tool sub type: Mouse
- Model: Conditional KO
- Conditional: Yes
- Genetic background: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110akinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Phenotype: Homozygous and heterozygous mice are phenotypically normal.
- Production details: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110Îąkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Additional notes: Homozygous and heterozygous mice are phenotypically normal.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: PIK3CA
References
- Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.
