Pik3ca-H1047R conditional Flp inducible

Contributors

Inventor Institute
Bart Vanhaesebroeck Ludwig Institute for Cancer Research
Cat. #: 158395
Research Fields: Cancer
Target: PIK3CA
Model: Conditional KO
Phenotype: Homozygous and heterozygous mice are phenotypically normal.
Conditional: Yes
Genetic background: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110akinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
Production details: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110Îąkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
Additional notes: Homozygous and heterozygous mice are phenotypically normal.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.

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