Skip to main content

#158395

Pik3ca-H1047R conditional Flp inducible

Cat. #158395

Pik3ca-H1047R conditional Flp inducible

Cat. #: 158395

Sub-type: Mouse

Availability: 8-10 weeks

Model: Conditional KO

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Bart Vanhaesebroeck

Institute: Ludwig Institute for Cancer Research

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Tool name: Pik3ca-H1047R conditional Flp inducible
  • Research fields: Cancer
  • Tool sub type: Mouse
  • Model: Conditional KO
  • Conditional: Yes
  • Genetic background: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110akinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
  • Phenotype: Homozygous and heterozygous mice are phenotypically normal.
  • Production details: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110Îąkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
  • Additional notes: Homozygous and heterozygous mice are phenotypically normal.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: PIK3CA

References

  • Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.