Raf-/- KO Mouse

The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues.

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151476
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
Target: Raf1+/- heterozygous knockout
disease: Cancer
Model: Knock-Out
Zygosity: Heterozygous
Phenotype: Homozygous Raf-1-/- mice die in embryogenesis.
Product description: The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues.
Conditional: No
Genetic background: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
Production details: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

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