Raf-1FF/FF knockin mouse

A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151477
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
Target: Raf1 Y340F Y341F (inactive mutant)
disease: Cancer
Model: Knock-In
Strain: C57BL/6
Product description: A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Additional notes: Genetic Background / Cross History : C57BL/6
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

MEK kinase activity is not necessary for Raf-1 function.

Related Tools for Mouse models

Cat. # Tool Name
151467 p23+/- Mouse Key Info View Tool
151478 caMEK1 Mouse Key Info View Tool
151442 Inva5b1 Mouse Key Info View Tool
151520 CDH5(PAC)CreERT2 Mouse Key Info View Tool
151551 Inva2b1 Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.