Cat. #152988
Krt5-Cre;Braff/f;Raf1f/f Mouse
Cat. #: 152988
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Atopic Dermatitis
Model: Transgenic
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Contributor
Inventor: Manuela Baccarini
Institute: University of Vienna
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Krt5-Cre;Braff/f;Raf1f/f Mouse
- Alternate name: RAF, CRAF, BRAF1, RAFB1, B-Raf, RAF/MEK/ERK signalling pathway, Cre-loxP recombination, Eczema
- Research fields: Developmental biology;Drug development;Genetics;Microbiology
- Tool sub type: Mouse
- Disease: Atopic Dermatitis
- Model: Transgenic
- Conditional: Yes
- Conditional description: Tissue-specific Cre expression driven by the human Keratin 5 promoter ablating both Braf and Raf1 alleles in the epidermis
- Description: The skin is the largest organ of the body protecting it from damage and hosting immune system cells. To function effectively as a barrier, the skin continually regenerates producing new cells to replace the old. If the regeneration process fails the skin may produce tumours, and disruption to the immune barrier can lead to autoimmune or allergic skin diseases such as psoriasis or atopic dermatitis. This mouse model has epidermis-specific Braf/Raf1 ablation, mediated by Cre expression in the basal epidermal layer and follicular keratinocytes using the Keratin 5 promoter. In normal conditions Braf and RAF1 operate independently to balance MAPK signalling. Removal of Braf and Raf1 in this model caused mice to develop an allergic disease similar to human atopic dermatitis, characterized by IgE responses (chemokines and Th2-type cytokines) with local and systemic inflammation.
- Genetic background: Krt5-Cre;Braff/f;Raf1f/f mice were generated by crossing Krt5-Cre;Braff/f with Krt5-Cre;Raf1f/f animals
- Zygosity: Homozygous for both floxed Braf and floxed Raf1 alleles
- Production details: Krt5-Cre;Braff/f;Raf1f/f mice were generated by crossing Krt5-Cre;Braff/f with Krt5-Cre;Raf1f/f animals
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Epidermis-specific double knockout of Braf and Raf1 alleles
References
- Raguz et al. 2016. Elife. 5:. PMID: 27431613.
- Epidermal RAF prevents allergic skin disease.
- Kern et al. 2013. Oncogene. 32(19):2483-92. PMID: 22733131.
- Essential, non-redundant roles of B-Raf and Raf-1 in Ras-driven skin tumorigenesis.
- Ehrenreiter et al. 2005. J Cell Biol. 168(6):955-64. PMID: 15753127.
- Raf-1 regulates Rho signaling and cell migration.
