#151562

JamC -/- Mouse

Cat. #151562

JamC -/- Mouse

Cat. #: 151562

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Neuropathy

Model: Knock-Out

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Ralf H. Adams

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: JamC -/- Mouse
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
  • Tool sub type: Mouse
  • Disease: Neuropathy
  • Model: Knock-Out
  • Conditional: No
  • Description: In vivo study of JAM-C knockout; in vivo study of spermiogenesis; in vivo study of neuronal networks and integrity
  • Genetic background: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
  • Phenotype: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
  • Zygosity: Heterozygous
  • Production details: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
  • Additional notes: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Junctional adhesion molecule-C (JAM-C)

References

  • Gliki et al. 2004. Nature. 431(7006):320-4. PMID: 15372036.
  • Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C.