#151556

UNG Mouse

Cat. #151556

UNG Mouse

Cat. #: 151556

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Tomas Lindahl

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: UNG Mouse
  • Cancer: Blood cancer
  • Cancers detailed: B cell lymphoma
  • Research fields: Cancer;Genetics;Immunology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Conditional: No
  • Description: In vivo study of UNG knockout and DNA mismatch mutation during DNA synthesis. Mice develop B cell lymphoma and are abnormally defective in their immune response. There are also mouse embryonic fibroblasts from this line available.
  • Genetic background: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
  • Zygosity: Homozygous
  • Production details: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Uracil-DNA Glycosylase (UNG)

References

  • Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
  • Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
  • Nilsen et al. 2000. Mol Cell. 5(6):1059-65. PMID: 10912000.
  • Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.