UNG Mouse

In vivo study of UNG knockout and DNA mismatch mutation during DNA synthesis. Mice develop B cell lymphoma and are abnormally defective in their immune response. There are also mouse embryonic fibroblasts from this line available.

Contributors

Inventor Institute
Tomas Lindahl Cancer Research UK, London Research Institute: Clare Hall Laboratories
Cat. #: 151556
Cancer types: Blood cancer
Research Fields: Cancer;Genetics;Immunology
Target: Uracil-DNA Glycosylase (UNG)
disease: Cancer
Zygosity: Homozygous
Product description: In vivo study of UNG knockout and DNA mismatch mutation during DNA synthesis. Mice develop B cell lymphoma and are abnormally defective in their immune response. There are also mouse embryonic fibroblasts from this line available.
Conditional: No
Production details: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.

Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.

Nilsen et al. 2000. Mol Cell. 5(6):1059-65. PMID: 10912000.

Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.

Related Tools for Mouse models

Cat. # Tool Name
151553 Pik3ca RBD mutant Mouse Key Info View Tool
151454 Bmx Cre ERT2 Mouse Key Info View Tool
151476 Raf-/- KO Mouse Key Info View Tool
151519 BRaf1 KI Mouse Key Info View Tool
151552 LFA1 -/- Mouse Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.