Chic1CBS-KO mouse

Inversion of Linx cluster of CTCF sites leads to Xist upregulation in cis. (A) The Linx locus, CTCF binding, and orientation of CTCF motifs associated with CTCF ChIP-seq peaks. The red and blue arrowheads indicate the orientation of the CTCF motif (orientated left or right, respectively). The targeted inversions Linx-25 kb-INV and Linx-51 kb-INV are indicated.
(F) (Up) RNA allelic ratios for Xist and the X-linked gene Atp7a. Each black dot corresponds to a single female embryo. Box-and-whisker plots indicate median, interquartile range and min/max values, with blue and red plots indicating paternally or maternally inherited alleles, respectively. (Down) Crosses used for analysis of RNA allelic ratios in female hybrid embryos inheriting the Mus musculus domesticus allele paternally (blue).