BRaf1 KI Mouse

Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways

Contributors

Inventor Institute
Catrin Pritchard University of Leicester
Cat. #: 151519
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Cell signaling and signal transduction;Genetics
Target: BRaf V600E (oncogenic mutant)
Model: Knock-In
Zygosity: Heterozygous
Alternate name: LSL B-RafV6E
Product description: Conditional knockin of oncogenic B-Raf V600E; in vivo study of oncogenic B-raf mutation and signalling pathways The LSL-B-Raf-V600E targeting vector, containing a loxP flanked minigene+polyA encoding wildtype exons 15 to 18, amd a loxP flanked resistance cassette, and a substitution of residue 600 (V to E) in the endogenous exon 15, was transfected into E14.1 ES cells. Properly targeted ES cells containing homologous recombination events were injected into C57BL6 balstocysts. Chimeric mice were backcrossed on the C57BL6 background.
Conditional: Yes
Conditional description: B-Raf gene with loxP sites flanking wildtype exons 15 to 18 (plus STOP) inserted before mutant exons 15 (V600E) to 18, enabling conditional Cre-mediate excision of wildtype exons and simultaneous knockin of mutant exons.
Genetic background: E14.1/C57BL6
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Mercer et al. 2005. Cancer Res. 65(24):11493-500. PMID: 16357158.

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