#151550

Integrin a6b4 Mouse

Cat. #151550

Integrin a6b4 Mouse

Cat. #: 151550

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Cancer

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Contributor

Inventor: Fiona Watt

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: Integrin a6b4 Mouse
  • Cancer: Skin cancer
  • Cancers detailed: Squamous cell carcinoma
  • Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Genetics;Stem cell biology
  • Tool sub type: Mouse
  • Disease: Cancer
  • Conditional: Yes
  • Conditional description: Conditional expression of integrins under involucrin promoter giving tissue-specific suprabasal epidermal expression.
  • Description: Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin. Ninety six percent of alpha6beta4 transgenic mice develop squamous cell carcinoma, compared to 44% wildtype. Of those mice developing tumours, 68% alpha6beta4 develop metastasis, compared to 8% wildtype. TGF-beta signalling is disrupted in alpha6beta4 transgenic mice.
  • Genetic background: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
  • Phenotype: Mice present phenotype very similar to human psoriasis disease.
  • Zygosity: Heterozygous
  • Production details: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Alpha6Beta4 Integrin

References

  • Owens DM et al, Journal of Cell Science, 2003, v116 pp3783-3791PMID: 12902406