V5-tagged form of JMJD6 U2OS cell line

Jumonji Domain Containing 6, Arginine Demethylase And Lysine Hydroxylase

Contributors

Inventor Institute
Yvan Canitrot Toulouse Tech Transfer
Cat. #: 161709
Tool sub type: Continuous
Cancer types: Sarcoma
Research Fields: Cancer
Organism: Human
Tissue: Bone
Gender: Female
Model: Knock-Out; Knock-In
Morphology: Epithelial
Growth properties: Adherent
Cancer Types In Detail: Osteosarcoma
Primary citation: Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.
Alternate name: V5-tagged form of JMJD6 deficient U2Os cell line
Product description: V5-tagged form of JMJD6 deficient U2OS cell line used to study the role of the JMJD6 enzyme in DNA maintenance following DNA damage
Gender: Female
CRISPR: Yes
Conditional: No
Production details: Gene inactivation by CRISPR-Cas9 and reintroduction of JMJD6 via stable expression of a plasmid coding for JMJD6
Receptors of note: Insulin like growth factor
Parental cell line: U2OS
Model description: JMJD6 knock out using CRISPR-Cas9 and knock in of a V5-tagged form of JMJD6
disease: Myasthenic Syndrome
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM medium supplemented with 10% foetal calf serum, sodium pyruvate and antibiotics (penicillin/streptomycin)
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: Parental U2OS cell line, JMJD6 WT cell lines, JMJD6 deficient U2OS cell line
Biosafety level: 1
References:

Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.

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