#153624

UB-UE1 Cell Line

Please Enter Your Email ID
Email ID

Cat. #153624

UB-UE1 Cell Line

Cat. #: 153624

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Mouse

Disease: Hearing loss

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Matthew C Holley

Institute: University of Bristol; University of Sheffield

Primary Citation: Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: UB-UE1 Cell Line
  • Alternate name: UB-UE1; UB/UE-1; University of Bristol/Utricular Epithelium-1; Utricular Epithelium cell line number 1
  • Research fields: Developmental biology;Drug development;Genetics
  • Parental cell: Pure utricular sensory epithelia of 2-day old immortomouse pups
  • Organism: Mouse
  • Disease: Hearing loss
  • Model: Immortalised Line
  • Conditional: Yes
  • Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the gamma interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
  • Description: Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, alpha9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro
  • Application: Inner ear development; Gene expression and function of inner ear-specific genes; In vitro screening for gene activation and promoter analysis; Ototoxicity studies (prescribed drugs and agents that ameliorate their affects)
  • Biosafety level: 1
  • Cellosaurus id: CVCL_D807

Applications

  • Application: Inner ear development; Gene expression and function of inner ear-specific genes; In vitro screening for gene activation and promoter analysis; Ototoxicity studies (prescribed drugs and agents that ameliorate their affects)

Handling

  • Format: Frozen
  • Volume: 1 ml
  • Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine,
  • Temperature: 33° C
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954
  • Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448