UB-UE1 Cell Line

Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions […]

Contributors

Inventor Institute
Matthew C Holley University of Bristol; University of Sheffield
Cat. #: 153624
Unit size: 1×10^6 cells / vial
Research Fields: Developmental biology;Drug development;Genetics
Organism: Mouse
Model: Immortalised Line
Primary citation: Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448
Alternate name: UB-UE1; UB/UE-1; University of Bristol/Utricular Epithelium-1; Utricular Epithelium cell line number 1
Product description: Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, alpha9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro
Conditional: Yes
Conditional description: Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the gamma interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the ImmortomouseTM (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Cellosaurus ID: CVCL_D807
Parental cell line: Pure utricular sensory epithelia of 2-day old immortomouse pups
disease: Hearing loss
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine,
Temperature: 33° C
Mycoplasma free: Yes
Biosafety level: 1
References:

Rivolta and Holley. 2002. J Neurobiol. 53:306-18. PMID: 12382283

Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954

Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448

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