UB-OC2 Cell Line

A conditionally immortal cell line derived from the mouse cochlea. The immortalizing gene was activated in organotypic cultures of auditory sensory epithelia at E13, the thirteenth day of embryonic development, before the hair cells had started to differentiate after their last mitoses. The cell line expresses characteristic hair cell markers including the transcription factor Brn3.1, […]

Contributors

Inventor Institute
Matthew C Holley University of Bristol; University of Sheffield
Cat. #: 153623
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Developmental biology;Drug development;Genetics
Organism: Mouse
Tissue: Developing organs of Corti
Model: Conditionally immortalised
Primary citation: Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783
Alternate name: Organ of Corti cell line number 2; UB/OC-2
Product description: A conditionally immortal cell line derived from the mouse cochlea. The immortalizing gene was activated in organotypic cultures of auditory sensory epithelia at E13, the thirteenth day of embryonic development, before the hair cells had started to differentiate after their last mitoses. The cell line expresses characteristic hair cell markers including the transcription factor Brn3.1, the a9 subunit of the acetylcholine receptor, the stereociliary protein fimbrin and the myosins VI and VIIA. UB/OC-2 cells have higher expression of Brn3.1, the a9AChR and myosin VIIa compared with UB/OC-1 at 33°C suggests that the former may have been immortalized at a later stage of differentiation and therefore represent committed hair cell precursors. This means that UB/OC-2 can be used specifically to study hair cell development at an early stage. At this stage they do not display the hair cell phenotype (cuticular plate, stereocilia, polarised organisation, etc.), but do express proteins specific to the hair cell transduction apparatus
Conditional: Yes
Conditional description: Proliferating cultures at 33°C in the presence of Î?IF and differentiating cultures at 39°C in the absence of Î?IF due to the temperature sensitivity of the immortalizing gene regulated by a Î?-interferoninducible promoter
Production details: Cells derived from C57/Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the Î?–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouseâ„? (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Cellosaurus ID: CVCL_D790
Parental cell line: Primary cultures of the developing organs of Corti of E13 embryonic Immortomouseâ„?
disease: Hearing loss
Format: Frozen
Storage conditions: Store in LN2 after a short period at -80° C
Shipping conditions: Dry ice
Growth medium: MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine
Temperature: 33° C
Atmosphere: 5% CO2
Storage medium: Pure Foetal Calf serum with 10% DMSO
Mycoplasma free: Yes
Biosafety level: 1
References:

Fritzsche et. al. Int J Mol Sci. 2022 May 21, 23(10):5780. PMID: 35628594

Clough et al. 2004. Biochem Biophys Res Commun. 324(1):372-81. PMID: 15465029

Jagger et al. 2000. J Physiol. 527(Pt 1):49-54. PMID: 10944169

Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081

Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783

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