U-251/EGFP_ LC3 – Mito DsRed

Astrocytoma cell line U-251 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.

Contributors

Inventor Institute
T.R. Santhosh Kumar Rajiv Gandhi Centre for Biotechnology
Cat. #: 161162
Research Fields: Cancer;Disease (not cancer);Metabolism
Organism: Human
Tissue: Brain
Gender: Male
Model: Transgenic
Morphology: Epithelial
Growth properties: Adherent
Primary citation: Lupitha SS, et.al. PMID: 32109534.
Product description: Astrocytoma cell line U-251 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.
Gender: Male
CRISPR: No
Conditional: No
Parental cell line: U-251MG
disease: Cancer
Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated).
Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: Growth medium + 10% DMSO
References:

Lupitha SS, et.al. PMID: 32109534.

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