#161156

U-251/EGFP_LC3 – mCherry_Smac

Cat. #161156

U-251/EGFP_LC3 – mCherry_Smac

Cat. #: 161156

Organism: Human

Tissue: Brain

Disease: Cancer

Model: Transgenic

£575.00

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Contributor

Inventor: T.R. Santhosh Kumar

Institute: Rajiv Gandhi Centre for Biotechnology

Primary Citation: Lupitha SS, et.al. PMID: 32109534.

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: U-251/EGFP_LC3 – mCherry_Smac
  • Parental cell: U-251MG
  • Organism: Human
  • Gender: Male
  • Tissue: Brain
  • Disease: Cancer
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Transgenic
  • Crispr: No
  • Conditional: No
  • Description: Astrocytoma cell line U-251 stably expressing both Smac ?‚– mCherry and EGFP-LC3 for use as a dual sensor for apoptosis and autophagy.

Handling

  • Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or RPMI 1640 with 2mM L-Glutamine and 10 Fetal Bovine serum.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry Ice
  • Storage medium: Growth medium + 10% DMSO
  • Storage conditions: Liquid Nitrogen
  • Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
  • Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
  • Cultured in antibiotics: No

References

  • Lupitha SS, et.al. PMID: 32109534.