Cat. #161156
U-251/EGFP_LC3 – mCherry_Smac
Cat. #: 161156
Organism: Human
Tissue: Brain
Disease: Cancer
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: T.R. Santhosh Kumar
Institute: Rajiv Gandhi Centre for Biotechnology
Primary Citation: Lupitha SS, et.al. PMID: 32109534.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: U-251/EGFP_LC3 – mCherry_Smac
- Research fields: Cancer;Disease (not cancer);Metabolism
- Parental cell: U-251MG
- Organism: Human
- Gender: Male
- Tissue: Brain
- Disease: Cancer
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Transgenic
- Crispr: No
- Conditional: No
- Description: Astrocytoma cell line U-251 stably expressing both Smac ? mCherry and EGFP-LC3 for use as a dual sensor for apoptosis and autophagy.
Handling
- Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or RPMI 1640 with 2mM L-Glutamine and 10 Fetal Bovine serum.
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: Growth medium + 10% DMSO
- Storage conditions: Liquid Nitrogen
- Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
- Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm? .
- Cultured in antibiotics: No
References
- Lupitha SS, et.al. PMID: 32109534.
