SW480 EGFP-emerin cell line clone 2

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: SW480 EGFP-emerin cell line clone 2
• Alternate name: SW480 GFP-emerin 2;
• Parental cell: SW480
• Clone: clone 2
• Organism: Human
• Tissue: Colon
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Genetically modified cell line
• Receptors of note: No
• Description: Colon cancer cell line stably transfected with EGFP-emerin. Suitable for studying the effect of the presence of lamin and/or emerin on cancer cell properties.
• Recommended controls: Same cell line stably transfected with EGFP not tagged to any other DNA

Target Details

• Target: Emerin
• Target background: Class II nuclear protein integral to the inner nucleear membrane which binds lamin A/C.

Handling

• Format: Frozen
• Passage number: Not known. Cells are high passage (>100) due to being a cancer cell line from a culture bank and then going through multiple passages to grow up the stable transfectants.
• Growth medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 100units/ml penicillin / 100ug/ml streptomycin
• Atmosphere: Without CO2
• Shipping conditions: Dry ice
• Storage medium: L15 (Leibovitz) medium with 2mM L-Glutamine, 10% FBS and 10% DMSO
• Storage conditions: Liquid Nitrogen
• Initial handling information: Continue to store at ?-70deg C. To bring up cells, defrost quickly in 37?C water bath and seed in petri dish/flask. Once cells have adhered to culture surface, change media to DMSO-free media.
• Subculture routine: Passage at 70-80% confluencey in the presence of 0.25% trypsin in 0.5mM EDTA/PBS. Incubate at 37?C, 5% Co2, humidified for 2 minutes. Split 1:4.
• Cultured in antibiotics: penicillin/streptomycin
• Characterisation tests: RT-PCR, IF and Western Blotting to confirm expression of lamin A
• Str profiling: Unpublished WB analysis available

References

• Unpublished