Cat. #161916
SK-MEL-24 Cell Line
Cat. #: 161916
Organism: Human
Tissue: Skin
Model: Tumourigenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lloyd J. Old, Toshitada Takahashi
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-MEL-24 Cell Line
- Cancer: Skin cancer
- Cancers detailed: Melanoma
- Organism: Human
- Gender: Male
- Tissue: Skin
- Donor: This cell line was established from a metastatic site (lymph node) in a 67-year-old Caucasian male with malignant melanoma.
- Morphology: Stellate
- Growth properties: Adherent
- Model: Tumourigenic
- Model description: Tumourigenic; forms malignant melanoma
- Products or characteristics of interest: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 2; PGM1, 1; PGM3, 2
- Description: K-MEL-24 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras.
- Application: 3D cell culture
- Biosafety level: 1
Target Details
- Target: Antigen expression: Blood Type O; Rh+; HLA A1, A2, B12, B14, Cw5
Applications
- Application: 3D cell culture
Handling
- Growth medium: EMEM supplemented with FBS to a final concentration of 15%
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Subculture routine: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking
- Str profiling: Amelogenin: X,YCSF1PO: 11D13S317: 12,13D16S539: 9,12D5S818: 9,13D7S820: 9,12TH01: 6,9TPOX: 11vWA: 15D3S1358: 15D21S11: 29,31.2D18S51: 16Penta_E: 11,12Penta_D: 9D8S1179: 14,15FGA: 22,23D19S433: 13,14D2S1338: 23
References
- Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.
