SK-LU-1 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: SK-LU-1 Cell Line
• Cancer: Adenocarcinoma
• Gender: Female
• Tissue: Lung
• Morphology: Epithelial
• Growth properties: Adherent
• Products or characteristics of interest: The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
• Description: SK-LU-1 is a human lung adenocarcinoma cell line, primary that displays epithelial morphology and grows in adherent culture. This cell line expresses mutant K-Ras (G12D) and has homozygous deletions in the CDH6 and CDKN2A genes. These cells do not express the enzyme telomerase reverse transcriptase (hTERT) and consequently lack telomerase activity. This correlates with significantly reduced tumourigenicity in vitro and in vivo. These cells, however, display characteristics of alternative telomere lengthening (ALT) mechanisms (i.e., heterogeneity of telomere lengths and the presence of distinct nuclear structures called ALT-associated promyelocytic leukaemia bodies). The SK-LU-1 cells do not form tumours when injected into immunocompromised mice.
• Biosafety level: 1
• Recommended controls: -

Target Details

• Target: Antigen expression: Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41

Handling

• Growth medium: EMEM suplemented with 10% FBS.
• Temperature: 37° C
• Shipping conditions: Dry Ice
• Storage medium: Complete growth medium, 95%; DMSO, 5%
• Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
• Characterisation tests: -
• Str profiling: D3S1358: 18;TH01: 7;D21S11: 29,30,30.2;D18S51: 18;Penta_E: 5;D5S818: 11;D13S317: 10;D7S820: 9;D16S539: 8;CSF1PO: 10;Penta_D: 10,13;Amelogenin: X;vWA: 16,17;D8S1179: 10;TPOX: 8,10;FGA: 21,22;D19S433: 15;D2S1338: 16,17

Related Tools

• Related tools: -

References

• Fogh J et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.
• Lehman et al. 1991. Cancer Research. 51: 4090-4096. PMID: 1855224.
• Brachner A et al. 2006. Cancer Research. 66: 3584-3592. PMID: 16585183.