Cat. #161934
SK-HEP-1 Cell Line
Cat. #: 161934
Availability: 8-10 weeks
Tissue: Liver; Ascites
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Germain Trempe, Jorgen Fogh
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SK-HEP-1 Cell Line
- Cancer: Adenocarcinoma
- Tissue: Liver; Ascites
- Donor: This cell line was established in 1971 from the ascites fluids of a 52-year old Caucasian male with adenocarcinoma of the liver.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: The cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. Eight markers are identified: del(1)(q21), der(1)t(1;2)(p36;q21), ?tdic(1;12)(p36;q24), ampl.t(4pter--->4q11::HSR::4q12--->4q24::HSR?::4q26--->4q35::2q15--->2pter), 13p+, 13p++, 14p+, t(14q15q), iso(14q), 19q+, del(3)(p14p24), 15p+. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 2 PGM3, 1
- Description: SK-HEP-1 is an immortal, human hepatic adenocarcinoma cell line that grows in adherent culture.
- Application: 3D cell culture; Cardiovascular disease research; Cancer research; High-throughput screening; Toxicology
- Biosafety level: 1
Applications
- Application: 3D cell culture; Cardiovascular disease research; Cancer research; High-throughput screening; Toxicology
Handling
- Growth medium: 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium then supplemented with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: XCSF1PO: 11,12D13S317: 8,12D16S539: 12D5S818: 10,13D7S820: 8,11TH01: 7,9TPOX: 9vWA: 14,17D3S1358: 16D21S11: 29,31D18S51: 13,15Penta_E: 13,21Penta_D: 13,14D8S1179: 13,14FGA: 17D19S433: 12,15.2D2S1338: 20,23
References
- Glasgow et al. 1984. Cancer research. 44(7):3022-3028. PMID: 6327032.
- Wang et al. 2012. Molecular cancer therapeutics. 11(4):864-72. PMID: 22351746.
