PC346C Cell line

Prostate cancer cell line derived from PDX (from transurethral resection of a primary prostate tumour); AR+, PSA+

Contributors

Inventor Institute
Wytske van Weerden Erasmus University Medical Centre
Cat. #: 161809
Cancer types: Genitourinary cancer
Research Fields: Cancer
Organism: Human
Tissue: Transurethral resection of the prostate (TURP)
Gender: Male
Primary citation: Marques et al. 2006. Eur Urol. 49(2):245-57. PMID: 16413664 ; Marques et al. 2010. PLoS One. 5(10):e13500. PMID 20976069 ; Marques et al. 2005. Int J Cancer. 117(2):221-229. PMID 15900601.
Product description: Prostate cancer cell line derived from PDX (from transurethral resection of a primary prostate tumour); AR+, PSA+
Gender: Male
Initial handling information: Protocols/SOPs available from inventor
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Prostate growth medium (PGM): DMEM-F12, 1X insulin-transferrin-selenium, 100 ng/mL fibronectin, 20 μg/mL fetuin, 0.01% BSA, 10 ng/mL epidermal growth factor, 50 ng/mL cholera toxin, 100µM phosphorylethanolamine, 0.6 ng/mL triiodothyronine, 0.1nM R1881, 10μM Y-27632 dihydrochloride, 0.5 µg/mL hydrocortisone and penicillin/streptomycin antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin). Supplemented with 2% FCS.
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: PGM+20S+7.5% DMSO
Biosafety level: 2

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