MHYO-LCL2 Human B-lymphoblast Cancer Cell Line

This cell line is a useful tool for reactivation of EBV-specific cytotoxic T-lymphocytes in vitro.

Contributors

Inventor Institute
Michal Hayun Rambam Health Care Campus
Cat. #: 160592
Tool sub type: Primary
Unit size: 1×10^6 cells / vial
Cancer types: Blood cancer
Research Fields: Cancer;Drug development;Immunology
Organism: Human
Tissue: Blood
Model: Tumour line
Growth properties: B-lymphoblast. Morphology: B-lymphoblastoid. Confirmed antigen expression: CD19, CD20, CD27, CD38, IgG. Confirmed cytokine secretion: IL-6, IL-10.
Cancer Types In Detail: AML;Leukaemia
Product description: The MHYO-LCL2 is an immortalised cancer cell line derived from a patient with acute myeloid leukaemia. The cell type is B-lymphoblast. This cell line is a useful tool for reactivation of EBV-specific cytotoxic T-lymphocytes in vitro. Relevant research areas include: Drug discovery, B-cell biology, B-cell development and transformation, and acute myeloid leukaemia. These cells are polyclonal as confirmed by IGH gene clonality assay.
Conditional: No
Production details: This cell line was spontaneously grown from peripheral blood drawn from a female acute myeloid leukaemia patient upon diagnosis. This line was established in culture four weeks after seeding the AML blast cells. The cells contain low EBV mRNA expression and secret EBV into media, confirming immortalisation and transformation. These cells are in suspension and mostly grow as aggregates.
Parental cell line: Novel patient-derived (female; acute myeloid leukaemia) cell line
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Cells have been grown in IScove Modified Dulbecco's Medium containing 10% foetal bovine serum and 1% pencicillin/streptomycin. Cells are grown at 37 degrees celsius and 5% CO2. Cultures were split at confluency 1:2 or 1:3 two or three times per week.

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