MET1 SCC Cell Line

Keratinocyte cell line representing the primary acantholytic carcinoma, that originated from an invasive and ultimately metastatic SCC.

Contributors

Inventor Institute
Irene Leigh Queen Mary University of London
Cat. #: 153539
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Model: Tumour line
Cancer Types In Detail: Skin;Skin cancer
Alternate name: SCC, Keratinocyte
Product description: Keratinocyte cell line representing the primary acantholytic carcinoma, that originated from an invasive and ultimately metastatic SCC. MET1 is derived from a clinical progression of a primary epidermal tumour from the back of a left hand through to an invasive and metastatic lesion. Histology of the original tumour (from which MET-1 was derived) showed an acantholytic squamous cell carcinoma. MET1 has been used to understand aggressive primary SCC after it has metastasised. It is an important cell line for this specific stage in SCC cancer progression. MET1 is the first cell line in a unique series of epidermal cell lines representing different stages of malignant transformation, that were derived from a single, adult, immunosuppressed individual. Four keratinocyte lines, PM1-4, established from forehead skin are here compared with 4 squamous cell carcinoma (SCC) lines, MET1-4, derived respectively from a primary cutaneous tumour, two local recurrences and a distant metastasis of invasive SCC. Despite altered growth properties, the PM lines retained many features of normal keratinocytes including keratin phenotype, differentiation capacity and non-tumorigenicity in athymic mice. In contrast, from early passage, the MET lines displayed markedly reduced growth requirements, abnormal differentiation, aberrant K18 expression and tumorigenicity in athymic mice. The abnormal keratin profile of individual MET lines closely reflected the keratin phenotype of the tumour of origin. Although unusual HPV types were identified in the original tissue, there was no evidence of persistent virus within any cell line and it appears that HPV is not critical for maintenance of the immortal phenotype. The PM lines were distinctly different from invasive SCC lines and are likely to be useful in studies focusing on mutations that are important in early neoplastic progression. The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process
Production details: MET1 was derived from a primary and invasive SCC excised from the back of the left hand
Additional notes: Difference Between Clones All derived from the same individual, but from varying stages in progression of an SCC: invasive, recurrent and, subsequently metastatic. Keratinocyte lines MET1, MET2, MET3 and MET4 were derived, respectively, from an SCC excised from the back of the left hand (MET1), from 2 recurrent SCC arising at the same site in rapid succession (MET2 and 3)and from metastatic SCC within left axillary lymph nodes biopsied following the third excision (MET4).
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Keratinocyte cell culture media supplemented with 10ng/ml EGF (Long term growth), DMEM/5% serum (Banking purposes)
Biosafety level: 1
References:

Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019.

The genomic landscape of cutaneous SCC reveals drivers and a novel azathioprine associated mutational signature.

Abikhair Burgo et al. 2018. JCI Insight. 3(17):. PMID: 30185657.

Abikhair Burgo et al. 2018. JCI Insight. 3(17):. PMID: 30185657.

Mekhdjian et al. 2017. Mol Biol Cell. 28(11):1467-1488. PMID: 28381423.

Proby et al. 2000. Exp Dermatol. 9(2):104-17. PMID: 10772384.

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