Cat. #162024
Melan-Oa1 cell line
Cat. #: 162024
Organism: Mouse
Tissue: Skin
Disease: Ocular Albanism
Model: Immortalised non-cancerous cell lines
£575.00
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Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melan-Oa1 cell line
- Organism: Mouse
- Tissue: Skin
- Disease: Ocular Albanism
- Morphology: Small, oval-fusiform
- Model: Immortalised non-cancerous cell lines
- Description: The melan-Oa1 line is an immortal melanocyte cell line derived from neonatal Oa1-/- 129/Sv mice. These cells lack the protein product of the ocular albinism type 1 gene, Oa1, an atypical G-protein coupled receptor found on the membranes of organelles including melanosomes. In humans the most common form of ocular albinism (Ocular albinism type 1) results from the loss of OA1 from retinal pigment epithelial cells, with a variable mild phenotype observed in epidermal melanocytes. However the loss of Oa1 results in macro-melanosomes which have an abnormal distribution towards the cell periphery. These cells are a useful model for ocular albinism type 1 and for the study of melanosome maturation and movement. A murine melanocyte line heterozygous for Oa1 (melan-Oa1+/-) is also available from CancerTools.org.
- Application: Melanocyte cell biology
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + Foetal bovine serum (FBS) (10%) + 12-0-tetradecanoyl phorbol acetate (TPA) (200 nM)
- Storage conditions: liquid N2
