#162014

Melan-In1 cell line

Cat. #162014

Melan-In1 cell line

Cat. #: 162014

Organism: Mouse

Tissue: Skin

Disease: Non-cancer

Model: Immortalised non-cancerous cell lines

£575.00

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Contributor

Inventor: Elena Sviderskaya

Institute: St. George's University London

Tool Details
Applications
Handling

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Melan-In1 cell line
  • Organism: Mouse
  • Tissue: Skin
  • Disease: Non-cancer
  • Morphology: Small, oval-fusiform
  • Model: Immortalised non-cancerous cell lines
  • Description: The melan-ln1, -ln2, ln-3 and ln-4 lines are immortal melanocyte cell lines derived from the skin of neonatal Mlphln/Mlphln null Ink4aArf -/- C57BL6/J mice. The leaden gene product (melanophilin, Mlph) is a Rab27a effector protein involved in melanosome transport and as such, these leaden null melanocytes display perinuclear clustering of pigmented melanosomes. These murine melanocytes are a model of the human disorder Griscelli syndrome in which patients present with hypopigmented skin and hair and immunodeficiencies. These melanocytes are a useful tool for studies of pigmentation genetics and transport of photoprotective eumelanin.
  • Application: Melanocyte cell biology

Applications

  • Application: Melanocyte cell biology

Handling

  • Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
  • Storage conditions: liquid N2