MEF ULK1/2 WT(SV40) Cell Line

This MEF ULK1/2 WT(SV40) Cell line can be used as a wild-type control with ULK1 knockout mouse embryonic fibroblasts to study ULK1-dependent processes.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152607
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Growth properties: Autophagy
Product description: The MEF ULK1/2 WT cell line can be used as a wild-type control with ULK1 and ULK2 cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized with SV40 large T-antigen.
Conditional: Yes
Production details: Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SV40.
Cellosaurus ID: CVCL_5A51
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 10% FCS + 2mM Glutamine
Subculture routine: Trypsinise with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10^4/cm² Cultures must be incubated in a humidified 5% CO₂/95% air incubator at 37°C
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.

Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.

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