MDA MB-231 sgRNA1 & 2 cell line
MDA-MB-231 sgRNA1 & 2 cell line ÂÂ
Contributors
| Inventor | Institute |
|---|---|
| Priya Srinivas | Rajiv Gandhi Centre for Biotechnology (RGCB) |
| Cat. #: | 161601 |
|---|---|
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Eipgenetic modification |
| Morphology: | Epithelial slightly elongated morphology |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast carcinoma |
| Primary citation: | https://doi.org/10.1101/2022.04.30.490082 |
| Product description: | The cells contain site-specific methylations in the BRCA1 promoter, which are analogous to the sites of methylation in breast cancer patients. Since, the direct effect of BRCA1 promoter hypermethylation in diagnosis and prognosis of breast and ovarian tumors has been largely obscure because of lack of proper in vitro and in vivo models these cell lines could be of potential research interest to others |
|---|---|
| Gender: | Female |
| CRISPR: | Yes |
| Conditional: | Yes |
| Conditional description: | conditional BRCA1 Hypermethylated cell line which gets reverted in the presence of tetracycline/doxycycline |
| Production details: | Cells with wildtype unmethylated BRCA1 background were infected with lentiviruses that encode for deadCas9- DNMT3A fusion protein and synthetic guide RNAs under the control of a tet-off promoter. After successful infection, the cells were then established following FACS sorting. |
| Additional notes: | 1?g/ml of doxycycline for 12 hours can stop the eGFP signal from pCL-CTIG and BRCA1 hypermethylation will be reverted. |
| Receptors of note: | Expression of BRCA1 only in the presence of doxycycline |
| Parental cell line: | MDA MB 234 |
| Model description: | BRCA1 hypermethylated cells developed using CRISPR-Cas9 technology |
| disease: | Cancer |
| Initial handling information: | Store in Liquid Nitrogen. Thaw in 10% DMEM with 1% Penicillin-Streptomycin |
|---|---|
| Format: | Frozen |
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry Ice |
| Growth medium: | DMEM medium supplemented with 10% FBS and 1% Penicillin-Streptomycin |
| Subculture routine: | Split the cells into 1:3 ratio when the cells reach 80% Confluency |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Storage medium: | 90% FBS + 1% Penicillin-Streptomycin + 10% sterile DMSO |
| Recommended controls: | MDA-MB-231 sgRNA Control cell line, MCF-7 sgRNA1 controls Control cell line , MCF-7 sgRNA2 Control cell line |
| Biosafety level: | 2 |
| References: |
https://doi.org/10.1101/2022.04.30.490082 |
|---|
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