MCF7/182R-6 Cell Line

The MCF7/182R-6 Cell line is a breast cancer cell line resistant to fulvestrant. Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152105
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Cancer cell line
Growth properties: Adherent
Cancer Types In Detail: Breast cancer;Fulvestrant resistant
Primary citation: Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-534. PMID: 7759159.
Alternate name: MCF-7/182R-6
Product description: The MCF7/182R-6 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). The MCF7/182R-6 cell line is a human breast cancer cell line established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 182,780 (fulvestrant) in 100 nM concentration. The cellular classification is epithelial, and their shape is polygonal. MCF7/182R-6 cells express oestrogen receptor alpha and do not express progesterone receptor. Treatment with the steroidal antioestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth is needed to find targeted treatments to resistant tumour cells and treatments that can inhibit or delay the emergence of resistance.
Gender: Female
Conditional: Yes
Production details: The MCF7/182R-6 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 182,780 in 100 nM concentration, see Lykkesfeldt et al (1995). The MCF7/182R-6 cells are grown in the presence of fulvestrant.
Cellosaurus ID: CVCL_W536
Parental cell line: MCF7 S0.5
Disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol red free DMEM/F12 (1:1) supplemented with 1% FCS, Glutamax 2,5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance.
Subculture routine: After thawing, dilute the cell suspension with sufficient medium and distribute 5 mL each into T25 flasks to achieve a seeding density of 0.5-1.0 x 10^4 / cm2. Place in a 37°C, 5% CO₂ incubator. Change medium after 24 hours to remove residual DMSO and then every 2-3 days. Subculture routine: Split 1:30 weekly with Trypsin-EDTA for detachment at 37 °C for 5 minutes. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37° C
Atmosphere: 5% CO2
Storage medium: 10% DMSO in FCS.
Mycoplasma free: Yes
Biosafety level: 1
References:

Thrane et al. 2015. Oncogene. 34:4199–4210. PMID: 25362855.

Sonne-Hansen et al. 2010. Breast Cancer Res Treat. 121(3):601-613. PMID: 19697122.

Frogne et al. 2008. Breast Cancer Res Treat. 114(2):263-275. PMID: 18409071.

Frankel et al. 2006. Breast Cancer Res Treat. 104(2):165-179. PMID: 17061041.

Frogne et al. 2005. Endocr Relat Cancer. 12(3):599-614. PMID: 16172194.

Nabha et al. 2005. Oncogene. 24(19):3166-3176. PMID: 15735693.

Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-534. PMID: 7759159.

Images

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MCF7 182R-6 Cell Line. Mid log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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