MCF10A EditR (Constitutive Cas9 expression) cell line

cells that stably express Cas9 nuclease

Contributors

Inventor Institute
Corrado Santocanale National University of Ireland Galway
Cat. #: 161756
Research Fields: Cancer
Organism: Human
Tissue: Breast
Gender: Female
Growth properties: Adherent
Primary citation: Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
Product description: MCF10AEditR cells that stably express Cas9 nuclease. The cell line is used to perform a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious doses of a CDC7i
Gender: Female
CRISPR: Yes
Production details: MCF10AEditR cells that stably express Cas9 nuclease were generated by lentiviral transduction of the Edit-R Cas9 plasmid and blasticidin-mediated selection.
Parental cell line: MCF10A
Model description: CRISPR
disease: Fibrocystic disease
Products or characteristics of interest: CRISPR drug screening
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM supplemented with 5% Horse serum, 100 ng/ml cholera toxin, 10 ?g/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 ?g/ml Streptomycin
Temperature: 37° C
Atmosphere: 5% CO2
Biosafety level: 1
References:

Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864

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