KPC Cell Line (mixed genetic background)

The KPC mouse is an established and clinically relevant model of pancreatic ductal adenocarcinoma (PDA) which develops many key features observed in human PDA including pancreatic intraepithelial neoplasia alongside a robust inflammatory reaction including exclusion of effector T cells.

Contributors

Inventor Institute
Jennifer Morton Cancer Research UK, Glasgow Beatson Institute
Cat. #: 153600
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Pancreatic cancer
Research Fields: Cancer;Drug development
Organism: Mouse
Model: Transgenic
Cancer Types In Detail: Pancreatic cancer ; Pancreatic ductal adenocarcinoma
Primary citation: Morton et al. 2010. Gastroenterology. 139(1):292-303. PMID: 20303350
Alternate name: PDAC cell line
Product description: This KPC cell line represents a useful model of pancreatic ductal adenocarcinoma. This cell line was established from a popular KPC mouse model (Hingorani et al. 2005). It has a mixed genetic background. Details on source mouse model The KPC mice from which the KPC cell is derived, are an establish clinically relevant model of PDA, that develop many of the key features of observed in human PDA such as pancreatic intraepithelial neoplasia. The KPC mouse contains a conditional point mutation in the transformation related protein 53 gene (TP53R172H), and a point mutation in the KRAS gene (KRASG12D) both of which generate non-functional proteins. A lox-stop-lox termination sequence is encoded upstream of both mutated genes to prevent expression in the absence of Cre recombinase. PDX1 (pancreatic and duodenal homeobox 1) is a transcription factor necessary for pancreatic development. The PDX1 promoter enables expression of Cre recombinase in acini, islet and duct cells of the pancreas. Cre-mediated recombination excises the two lox-stop-lox termination sequences and enables expression of both oncogenes: KRASG12D and TP53R172H in pancreatic tissue. Tissues not expressing Cre recombinase remain functionally heterozygous of the KRAS and TRP53 loci. KPC cell line The KPC cell line was derived from a KPC model that has a mixed genetic background. This cell line retains the mutations seen in the parental KPC murine model. This KPC model can be used for PDA modelling, biomarkers investigation, genetics, and metastasis studies as well as drug discovery and development.
Conditional: No
Additional notes: These cells are from mice with a mixed genetic background
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM +10% FCS, 1% L-Glut and 1% Pen/strep. Revival might be slow.
Temperature: 38 ° C
Mycoplasma free: Yes
Biosafety level: 1
References:

Li et al. 2014. Gastroenterology. 146(5):1386-96.e1-17. PMID: 24462734.

Morton et al. 2010. Gastroenterology. 139(1):292-303. PMID: 20303350

Hingorani et al. 2005. Cancer Cell. 7(5):469-83. PMID: 15894267.

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