iPSC healthy control for neuronal differentiation (F002.1A.13) cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: iPSC healthy control for neuronal differentiation (F002.1A.13) cell line
• Research fields: Genetics;Neurobiology;Stem cell biology
• Parental cell: Human fibroblasts
• Organism: Human
• Tissue: Fibroblast
• Disease: Cerebellar diseases
• Growth properties: Healthy control
• Model: Stem Cells
• Conditional: No
• Description: Healthy control â?‚€?‚“ optimized for cerebellar neurons differentiation. Generation of electrophysiologically active GABAergic and glutamatergic cerebellar neurons in long-term cultures without co-culturing with other cell types. Relevant model for drug screening and for study specific pathways involved with the development of cerebellar diseases such as ataxias, a group of disorders that affect many children and adults worldwide.
• Production details: Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female healthy donor.

Target Details

• Target: Healthy control

Handling

• Format: Frozen
• Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTM1 Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3â?‚€?‚“4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 â?‚—ĚšC in a humidified 5% CO2 incubator.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice