HT-144 Cell Line

HT-144 is a malignant human melanoma cell line that displays aneuploid fibroblastic morphology and grows in adherent tissue culture. This cell line has been reported to be nonpermissive for human cytomegalovirus (HCMV). HT-144 cells form xenograft tumours when injected into immunocompromised mice. These cells contain a mutation in the ATM gene, resulting in the expression of a […]

Contributors

Inventor Institute
Jorgen Fogh Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161928
Cancer types: Skin cancer
Tissue: Subcutaneous tissue
Morphology: Aneuploid fibroblastic
Growth properties: Adherent
Cancer Types In Detail: Melanoma
Primary citation: Fogh et al. 1977. Journal of the National Cancer Institute. 58: 209-214. PMID: 833871.
Product description: HT-144 is a malignant human melanoma cell line that displays aneuploid fibroblastic morphology and grows in adherent tissue culture. This cell line has been reported to be nonpermissive for human cytomegalovirus (HCMV). HT-144 cells form xenograft tumours when injected into immunocompromised mice. These cells contain a mutation in the ATM gene, resulting in the expression of a truncated protein, which causes increased sensitivity to UVB and ionising radiation compared to other melanoma cell lines. The HT-144 cells also express mutant B-Raf (V600E).
Products or characteristics of interest: Karyotype – (P15) hypotriploid to hypertriploid (+A, +B, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, minutes, secondary constrictions, breaks, large submetacentric, subtelocentric and minute markers. Tumorigenic – Yes, in nude mice; forms anaplastic malignant tumor consistent with melanoma; tumors also form in steroid treated hamsters. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 2; PGM1, 1-2; PGM3, 1
Shipping conditions: Dry Ice
Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% DMSO
Biosafety level: 1
References:

Frohlich et al. 2023. Cancer Research Communications. 3(9). PMID: 37674529.

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