Cat. #158385
Histone deacetylase 1/2 (HDAC 1/2) double knockout mouse embryonic stem cell line
Cat. #: 158385
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Tissue: Embryo
Model: Knock-Out
£575.00
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Contributor
Inventor: Shaun Cowley
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Histone deacetylase 1/2 (HDAC 1/2) double knockout mouse embryonic stem cell line
- Alternate name: RPD3L1
- Research fields: Developmental biology
- Tool sub type: Continuous
- Parental cell: E14 ES cell line expressing an inducible Cre/Estrogen Receptor (CreER) construct from the endogenous ROSA26 locus
- Tissue: Embryo
- Model: Knock-Out
- Conditional: Yes
- Conditional description: To induce Hdac1/2 deletion, cells were cultured for 24 h in the presence of OHT (1 ÄÂÄ?M) to activate the CreER fusion protein.
- Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Histone deacetylases (HDAC) 1 and 2 are highly similar enzymes that help regulate chromatin structure as the core catalytic components of corepressor complexes. Although tissue-specific deletion of HDAC1 and HDAC2 has demonstrated functional redundancy, germ-line deletion of HDAC1 in the mouse causes early embryonic lethality, whereas HDAC2 does not.
- Production details: E14 ES cells expressing a CreER fusion protein from the ROSA26 locus were used to generate Hdac1Lox/Lox; Hdac2Lox/Lox; CreER conditional knockout ES cells, using multiple rounds of gene targeting
- Recommended controls: E14 ES parental line
Target Details
- Target: Histone deacetylase 1/2
Applications
- Application notes: Loss of exon 2 via the conditional KO disrupts the ORF of both HDAC1 and HDAC2 such that a premature stop codon is introduced in exon 3. Following this deletion of exon 2 a further 4?Â5 days of culture are required before HDAC1 and HDAC2 protein levels are reduced below 10% of those of control cells. HDAC1/2 are necessary for accurate chromosome segregation. Significantly for the maintenance of stem cell self-renewal, we detected a reduction in the expression of the pluripotent transcriptio...
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Jamaladdin et al. 2014. Proc Natl Acad Sci U S A. 111(27):9840-5. PMID: 24958871.
