Cat. #152597
E3 Cell Line
Cat. #: 152597
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Breast
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joyce Taylor-Papadimitriou ; Joy Burchell
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: E3 Cell Line
- Cancer: Gynaecologic cancer
- Cancers detailed: Mammary
- Research fields: Cancer;Drug development;Immunology
- Tool sub type: Continuous
- Parental cell: 410.4
- Organism: Mouse
- Tissue: Breast
- Disease: Cancer
- Model: Immortalised Line
- Description: The E3 cell line was developed to examine the possibility of using the MUC1 gene and its products in active immunization against breast and other carcinomas. The human MUC1 gene codes for a type I membrane glycoprotein that is normally expressed on the apical surface of most glandular epithelial cells, but which is upregulated and under- or differently glycosylated in carcinomas; these differences in glycosylation lead to the exposure of novel epitopes which are not found on the normally processed mucin and can therefore be used as targets.
- Production details: The E3 cell line was developed from the 410.4 murine mammary cell line by transfection of the hygromycin resistance gene with the MUC1 gene.
- Cellosaurus id: CVCL_W345
Target Details
- Target: hMUC1, hygromycin resistance gene
Handling
- Format: Frozen
- Growth medium: DMEM, 10S , 400??g/ml hygromycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
- Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
- Smith et al. 1999. Immunology. 97(4):648-55. PMID: 10457219.
- Expression of B7.1 in a MUC1-expressing mouse mammary epithelial tumour cell line inhibits tumorigenicity but does not induce autoimmunity in MUC1 transgenic mice.
- Lalani et al. 1991. J Biol Chem. 266(23):15420-6. PMID: 1714457.
- Expression of the gene coding for a human...
