#157985

DLKP-I cell line

Cat. #157985

DLKP-I cell line

Cat. #: 157985

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Lymph Node

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Martin Clynes

Institute: Dublin City University

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: DLKP-I cell line
  • Cancer: Lung cancer
  • Cancers detailed: Lung carcinoma
  • Research fields: Cancer;Drug development
  • Tool sub type: Continuous
  • Parental cell: DLKP
  • Organism: Human
  • Tissue: Lymph Node
  • Disease: Cancer
  • Growth properties: monolayer
  • Description: Sub types of lung carcinoma with well-defined morphology and characteristic studies
  • Production details: Single cell cloning from DLKP parental population
  • Biosafety level: 1
  • Recommended controls: DLKP parental line
  • Cellosaurus id: CVCL_H738

Applications

  • Application notes: Intermediate sub-population cloned at NCTCC fromDLKPP5, about 25% ofDLKPshowDLKP-I morphology. Interconversion between DLKP-M/DLKP-SQ and DLKP-I, tetraploid, fold resistance compared to DLKP: 0.5 Adr; 0.65 Vcr; 0.6 VP-16. Immunocytochemistry: cytokeratin (all) -; GFAP-; vimentin+; neurofilament+; P-gp-; a2-integrin+; desmin-; 703D4+; 704A1+.

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F12, 5% FCS. Split 1:5, medium change 2-3 times weekly. Freezing method: 10% DMSO in FBS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Keenan et al. 2012. Exp Cell Res. 318(5):593-602. PMID: 22281030.