CHO DC-SIGN-DeltaCRD cell line

Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens […]

Contributors

Inventor Institute
Alessandra Cambi Radboud University Medical Center
Cat. #: 160719
Unit size: 1×10^6 cells / vial
Research Fields: Cell signaling and signal transduction;Immunology
Organism: Hamster
Tissue: Ovary
Alternate name: DC-SIGN-Î?‚”CRD
Product description: Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens like Mycobacterium tuberculosis and Candida albicans.
Conditional: No
Production details: CHO cell lines stably expressing DC-SIGN-Î?‚”CRD were established by Lipofectamin 2000 transfection, and were cultured in Hamâ?‚€?‚™s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5â?‚€?‚‰mg/ml of the aminoglycoside antibiotic G418.
Parental cell line: CHO
disease: HIV-1
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Hamâ?‚€?‚™s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5â?‚€?‚‰mg/ml of the aminoglycoside antibiotic G418.
References:

Te Riet et al. 2017. Sci Rep. 7(1):6713. PMID: 28751750

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