Cat. #153289
FH-/- CL 1 Cell Line
Cat. #: 153289
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Mouse
Tissue: Kidney
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eyal Gottlieb
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: FH-/- CL 1 Cell Line
- Alternate name: Fumarate hydratase, FH, fumarase, Fh1, Hereditary leiomyomatosis and renal cell cancer, HLRCC
- Research fields: Cancer;Drug development;Metabolism
- Organism: Mouse
- Tissue: Kidney
- Disease: Cancer
- Model: Knock-Out
- Conditional: No
- Description: Fumarate hydratase – which can be found in either a mitochondrial or cytosolic form - is an enzyme that catalyzes the reversible hydration and dehydration of fumarate to malate. Signal sequences located in the protein dictate the subcellular location of each FH isoenzyme. The cytosolic form is involved in the metabolism of amino acids and fumarate and the mitochondrial form is involved in the Krebs Cycle (tricarboxylic acid cycle or the citric acid cycle). Germline mutations of FH are responsible for hereditary leiomyomatosis and renal-cell cancer (HLRCC)1.
- Biosafety level: 1
- Cellosaurus id: CVCL_HG01
Target Details
- Target: Fumarate Hydratase
Handling
- Format: Frozen
- Growth medium: Cells can be maintained in DMEM supplemented with 10% FBS, 2 mmol/l glutamine, 1 mmol/l pyruvate and 50 mg/l uridine. However, the inventor advises to use more physiological conditions for experiments, such as Plasmax, available on our website.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: FH fl/fl Cell Line ; FH-/- CL 19 Cell Line
References
- McCarthy et al. 2007. J Gene Med. 9(6):511-20. PMID: 17471586.
- Human osteocalcin: a strong promoter for nitric oxide synthase gene therapy, with specificity for hormone refractory prostate cancer. Frezza et al. 2011. Nature. 477(7363):225-8. PMID: 21849978.
- Haem oxygenase is synthetically lethal with the tumour suppressor fumarate hydratase.
- Pollard et al. 2007. Cancer Cell. 11(4):311-9. PMID: 17418408.
- Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway.
