Cat. #161633
1.2B4 cell line
Cat. #: 161633
Sub-type: Hybrid cell line
Organism: Human
Tissue: Pancreas
Disease: Diabetes
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Flatt and Neville McClenaghan
Institute: Ulster University
Primary Citation: Nishimura N et. al. 1993. Int J Pancreatol 13: 31-41 PMID: 8454916.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 1.2B4 cell line
- Research fields: Disease (not cancer)
- Tool sub type: Hybrid cell line
- Parental cell: HuP-T3 cell line
- Organism: Human
- Tissue: Pancreas
- Disease: Diabetes
- Morphology: Epitheloid
- Growth properties: Adherent
- Model: Transgenic
- Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line
- Crispr: No
- Description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line. 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host.
- Application: producing pure insulin secreting cells when stimulated
- Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
Applications
- Application: producing pure insulin secreting cells when stimulated
Handling
- Format: Frozen
- Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm� can be expected.
- Mycoplasma free: Yes
- Str profiling: STR-PCR Data:Amelogenin: X,YCSF1PO: 10D13S317: 9,11D16S539: 10,13D5S818: 10,14D7S820: 12THO1: 9TPOX: 8,12vWA: 18
References
- Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334
