Cat. #151414
Anti-Ufd1 [5e2]
Cat. #: 151414
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Ufd1
Class: Monoclonal
Application: ELISA ; IF ; IP ; WB
Reactivity: Human ; Rat ; Mouse ; Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Ufd1 [5e2]
- Research fields: Cancer;Cell biology
- Clone: 500
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 40 kDa
- Strain: Balb/c
- Reactivity: Human ; Rat ; Mouse ; Xenopus laevis
- Host: Mouse
- Application: ELISA ; IF ; IP ; WB
- Description: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
- Immunogen: Full length mouse Ufd1 expressed in E. coli
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
- Recommended controls: HeLa or HEK293 cell lysate.
Target Details
- Target: Ufd1
- Molecular weight: 40 kDa
- Tissue cell line specificity: HeLa or HEK293 cell lysate.
- Target background: The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis.
Applications
- Application: ELISA ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
- The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
- Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
- Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
- Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
- A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
