Cat. #151199
Anti-SIGLEC1 [HSn 7D2]
Cat. #: 151199
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Sialoadhesin domains 1-4 (SIGLEC1)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; RIA ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Paul Crocker
Institute: University of Dundee
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-SIGLEC1 [HSn 7D2]
- Alternate name: CD169, Sialoadhesin 1
- Research fields: Cancer;Cell biology;Immunology
- Clone: HSn 7D2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; RIA ; WB
- Description: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
- Immunogen: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Human spleen (IHC), human peripheral blood monocytes (FACS) or MCF7 cells (IF)
Target Details
- Target: Sialoadhesin domains 1-4 (SIGLEC1)
- Tissue cell line specificity: Human spleen (IHC), human peripheral blood monocytes (FACS) or MCF7 cells (IF)
- Target background: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
Applications
- Application: ELISA ; FACS ; IHC ; RIA ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Puryear et al. 2013. PLoS Pathog. 9(4):e1003291. PMID: 23593001.
- Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
- Rempel et al. 2008. PLoS One. 3(4):e1967. PMID: 18414664.
- Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
- Pack et al. 2008. Immunology. 123(3):438-46. PMID: 17944899.
- DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
- Hartnell et al. 2001. Blood. 97(1):288-96. PMID: 11133773.
- Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
- Steiniger et al. 1997. Immunology. 92(2):307-16. PMID: 9415041.
- The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
