Anti-PSMA5 [AH1.1]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-PSMA5 [AH1.1]
• Research fields: Cell biology
• Clone: AH1.1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human ; Xenopus laevis
• Host: Mouse
• Application: FACS ; IHC ; IP ; WB
• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.
• Immunogen: Sub-unit 5a
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: Proteasome complex; subunit 5a (PSMA5)
• Target background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.

Applications

• Application: FACS ; IHC ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Liu et al. 2014. J Clin Invest. 124(5):2059-70. PMID: 24691443.
• Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
• Kley et al. 2012. Brain. 135(Pt 9):2642-60. PMID: 22961544.
• Pathophysiology of protein aggregation and extended phenotyping in filaminopathy.
• Yi et al. 2010. Cell Tissue Res. 341(2):325-40. PMID: 20526895.
• Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization.
• Arlt et al. 2009. Oncogene. 28(45):3983-96. PMID: 19734940.
• Increased proteasome subunit protein expression and proteasome activity in colon cancer relate to an enhanced activation of nuclear factor E2-related factor 2 (Nrf2).