Cat. #153180
Anti-Phospho Endosulfine [DF1.1]
Cat. #: 153180
Sub-type: Primary antibody
Unit size: 100 ug
Target: Phospho Endosulfine
Class: Monoclonal
Application: WB
Reactivity: Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Phospho Endosulfine [DF1.1]
- Research fields: Neurobiology
- Clone: DF1.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 14 kDa
- Reactivity: Xenopus laevis
- Host: Mouse
- Application: WB
- Description: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.Endosulfine is highly phosphorylated in Xenopus CSF egg extracts but de-phosphorylated when the eggs enter interphase.
- Immunogen: Synthetic peptide - KGQKYFD-Sp-GDYNMAK
- Recommended controls: Xenopus CSF (cytostatic factor extract)
Target Details
- Target: Phospho Endosulfine
- Molecular weight: 14 kDa
- Tissue cell line specificity: Xenopus CSF (cytostatic factor extract)
- Target background: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.Endosulfine is highly phosphorylated in Xenopus CSF egg extracts but de-phosphorylated when the eggs enter interphase.
Applications
- Application: WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
