Cat. #151126
Anti-Keratin8 [LP3K]
Cat. #: 151126
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Keratin 8
Class: Monoclonal
Application: IHC ; IF ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Birgit Lane
Institute: Queen Mary University of London
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Keratin8 [LP3K]
- Clone: LP3K
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 52 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IHC ; IF ; WB
- Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.
- Immunogen: Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes.
- Immunogen uniprot id: P05783
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Human skin
Target Details
- Target: Keratin 8
- Molecular weight: 52 kDa
- Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.
Applications
- Application: IHC ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.
- Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.
- Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.
- The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.
- Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.
- Keratin antigens in differentiating skin.
