#151126

Anti-Keratin8 [LP3K]

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Cat. #151126

Anti-Keratin8 [LP3K]

Cat. #: 151126

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Keratin 8

Class: Monoclonal

Application: IHC ; IF ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Birgit Lane

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Keratin8 [LP3K]
  • Research fields: Cell signaling and signal transduction
  • Clone: LP3K
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 52 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF ; WB
  • Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.
  • Immunogen: Sonicated cytoskeleton fractions (insoluble in 1% Nonidet-P40) from SV40 transformed neonatal keratinocytes.
  • Immunogen uniprot id: P05783
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Human skin

Target Details

  • Target: Keratin 8
  • Molecular weight: 52 kDa
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas. Keratin 8 is the tissue polypeptide antigen (TPA) reported to be present in the serum of cancer patients.

Applications

  • Application: IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Waseem et al. 2004. Biochemistry. 43(5):1283-95. PMID: 14756564.
  • Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability.
  • Godsave et al. 1986. J Embryol Exp Morphol. 97:201-23. PMID: 2432146.
  • The appearance and distribution of intermediate filament proteins during differentiation of the central nervous system, skin and notochord of Xenopus laevis.
  • Lane et al. 1985. Ann N Y Acad Sci. 455:241-58. PMID: 2417515.
  • Keratin antigens in differentiating skin.