Anti-Haemoglobin Scavenger Receptor [GHI/61]

Monoclonal antibody which recognises circulating blood monocytes and the large majority of tissue macrophages, without labelling granulocytes or lymphocytes.

Contributors

Inventor Institute
Karen Pulford University of Oxford
Cat. #: 151380
Tool sub type: Primary antibody
Unit size: 100 ug
Research Fields: Immunology;Tissue-specific biology
Application: FACS ; IHC ; IF ; IP ; Fn ; WB
Target: Haemoglobin Scavenger Receptor (M130; CD163)
Reactivity: Human
Clone: GHI/61
Host: Mouse
Class: Monoclonal
Product description: Monoclonal antibody which recognises circulating blood monocytes and the large majority of tissue macrophages, without labelling granulocytes or lymphocytes.
Conjugation: Unconjugated
Isotype: IgG1
Immunogen: Hairy cell leukaemia cells
Immunogen Uniprot ID: Q86VB7
Myeloma used: P3/NS1/1-Ag4.1
Target background: Anti-haemoglobin scavenger receptor labels the majority of monocytes and tissue macrophages. It recognises a membrane glycoprotein with molecular weight 130,000 (reduced) and 110,000 (unreduced). The glycoprotein antigen is expressed exclusively on the cell surface of human monocytes and macrophages, which evolves predominantly in the late phase of inflammation. The glycoprotein GHI/61 binds to CD163, a transmembrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It is a receptor for the haemoglobin-haptoglobin complex. CD163 expression on monocytes and macrophages changes as these cells differentiate, as such GHI/61 can detect levels of differentiated monocytes and macrophages. CD163 has high affinity for the haemoglobin-haptoglobin complex and upon ligand binding, leads to the production of IL-6 and IL-10. GHI/61 monoclonal antibody was created to label monocytes and macrophage with increased specificity, given similar antibodies frequently label lymphocytes etc., or only recognise a particular subset of macrophages.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
Shipping conditions: Dry ice
References:

Salerno-Goncalves et al. 2019. PLoS Negl Trop Dis. 13(8):e0007650. PMID: 31412039.

Amini et al. 2019. BMC Cancer. 19(1):316. PMID: 30953461.

Schulz et al. 2019. Sci Rep. 9(1):1925. PMID: 30760760.

Silva et al. 2019. Front Immunol. 10:1508. PMID: 31333653.

Gruenbacher et al. 2019. Front Immunol. 10:1870. PMID: 31447857.

Moura et al. 2012. Eur J Immunol. 42(11):2925-36. PMID: 22851198.

CD163 favors Mycobacterium leprae survival and persistence by promoting anti-inflammatory pathways in lepromatous macrophages.

Moniuszko et al. 2006. Clin Vaccine Immunol. 13(6):704-7. PMID: 16760331.

Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants.

Mller et al. 2002. Blood. 99(1):378-80. PMID: 11756196.

Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma.

Law et al. 1993. Eur J Immunol. 23(9):2320-5. PMID: 8370408.

A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.

Pulford et al. 1992. Immunology. 75(4):588-95. PMID: 1592433.

A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4.

Related Tools for Antibodies

Cat. # Tool Name
151016 Anti-CyclinA [E72.1] Key Info View Tool
151026 Anti-FCGR1 [10.1] mAb Key Info View Tool
151029 Anti-HSVICP8 [11E2] Key Info View Tool
151031 Anti-RuvA [RuvA 12C6] Key Info View Tool
151038 Anti-Cytochrome P450 4A2, 4A3 [Clo4] Key Info View Tool

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.