Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types
| Inventor | Institute |
|---|---|
| Lynda Partridge | University of Sheffield |
| Cat. #: | 153598 |
|---|---|
| Unit size: | 100 ug |
| Research Fields: | Cell biology;Developmental biology |
| Application: | ELISA ; FACS |
| Target: | Filamentous Actin |
| Reactivity: | Chicken ; Human ; Mouse ; Rabbit |
| Clone: | NH3 |
| Host: | Mouse |
| Class: | Monoclonal |
| Alternate name: | Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody |
|---|---|
| Product description: | Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types. Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells. |
| Conjugation: | Unconjugated |
| Isotype: | IgM kappa |
| Immunogen: | Human monocytes and the human monocyte-like cell line, U937. |
| Immunogen Uniprot ID: | P60709 |
| Target background: | Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells. |
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| Format: | Liquid |
|---|---|
| Concentration: | 1mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | -15° C to -25° C |
| Shipping conditions: | Dry ice |
| References: |
Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163 |
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