#151374

Anti-CD79a [HM57] mAb

Cat. #151374

Anti-CD79a [HM57] mAb

Cat. #: 151374

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: CD79a (mb1)

Class: Monoclonal

Application: FACS ; IHC ; IP ; WB

Reactivity: Bovine ; Chicken ; Horse ; Human ; Guinea Pig ; Mouse ; Opossum ; Pig ; Primate ; Rat ; Rabbit

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jacqueline Cordell

Institute: University of Oxford

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-CD79a [HM57] mAb
  • Alternate name: CD79a Molecule; Membrane-Bound Immunoglobulin-Associated Protein; Surface IgM-Associated Protein; MB-1 Membrane Glycoprotein; Ig-Alpha; IGA; CD79a Antigen; MB1
  • Research fields: Cancer;Cell signaling and signal transduction;Immunology;Stem cell biology
  • Clone: HM57
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Bovine ; Chicken ; Horse ; Human ; Guinea Pig ; Mouse ; Opossum ; Pig ; Primate ; Rat ; Rabbit
  • Host: Mouse
  • Application: FACS ; IHC ; IP ; WB
  • Description: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.
  • Immunogen: Synthetic peptide of 14 amino acids representing residues 202-216 of the human mb-1 cDNA sequence.
  • Isotype: IgG1
  • Myeloma used: P3/NS1/1-Ag4.1

Target Details

  • Target: CD79a (mb1)
  • Target background: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling. CD79a is widely used as an adjunct to CD20 as a biomarker for normal and neoplastic B-cells in tissues sections.

Applications

  • Application: FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Mason et al. 1995. Blood. 86(4):1453-9. PMID: 7632952.
  • CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples.
  • Jones et al. 1993. J Immunol. 150(12):5429-35. PMID: 8515069.
  • Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
  • Mason et al. 1992. Eur J Immunol. 22(10):2753-6. PMID: 1396979.
  • The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.
  • Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
  • The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
  • van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
  • The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.