#152483

Anti-CD49b [16B4]

Cat. #152483

Anti-CD49b [16B4]

Cat. #: 152483

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-5 days

Target: CD49f (Integrin a2+b1)

Class: Monoclonal

Application: ELISA ; FACS ; IHC ; IF ; IP

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Martin Humphries

Institute: University of Manchester

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CD49b [16B4]
  • Alternate name: Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor; Alpha-2 Subunit; Human Platelet Alloantigen System; Platelet Glycoprotein GPIa; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR
  • Research fields: Cell biology;Cell signaling and signal transduction;Immunology
  • Clone: 16B4
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; IF ; IP
  • Description: CD49b is an integrin alpha subunit which makes up half of the Î?2Î?1 integrin duplex. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling.
  • Immunogen: Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
  • Isotype: IgG1
  • Myeloma used: P3X63Ag8.653
  • Recommended controls: In IF, this antibody gave a positive signal in HepG2 cells.

Target Details

  • Target: CD49f (Integrin a2+b1)
  • Tissue cell line specificity: In IF, this antibody gave a positive signal in HepG2 cells.
  • Target background: CD49b is an integrin alpha subunit which makes up half of the a2?1 integrin duplex. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling.

Applications

  • Application: ELISA ; FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Suarez et al. 2015. Arch Dermatol Res. 307(2):115-33. PMID: 25322916.
  • Menhofer et al. 2014. Cardiovasc Res. 104(2):303-14. PMID: 25239826.
  • Identification of biomarkers involved in differential profiling of hypertrophic and keloid scars versus normal skin.
  • In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor.
  • Tuckwell et al. 2000. Biochem J. 350 Pt 2:485-93. PMID: 10947963.
  • Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a Fnly important region within alpha2beta1.
  • Sincock et al. 1999. J Cell Sci. 112 ( Pt 6):833-44. PMID: 10036233.
  • PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
  • Fitter et al. 1999. Biochem J. 338 ( Pt 1):61-70. PMID: 9931299.
  • Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.