Anti-A. aeolicus BPL/BioID2 [SS QD1]

Monoclonal antibody used in the BioID method to detect protein-protein interactions.

Contributors

Institute
A*STAR Accelerate Technologies Pte Ltd
Cat. #: 153486
Unit size: 100 ug
Research Fields: Tags and cell markers
Application: IF ; WB
Target: BPL R40G
Reactivity: Aquifex aeolicus
Clone: SS QD1
Host: Mouse
Class: Monoclonal
Alternate name: BPL antibody, BioID2 antibody, Anti-BPL, Anti-BioID
Product description: Monoclonal antibody used in the BioID method to detect protein-protein interactions. Background and Research Application BioID2 is a second-generation biotin protein ligase (BPL) that can be used to identify protein-protein interactions. In the BioID method a promiscuous biotin protein ligase (BPL) is fused to a protein of interests and expressed in vivo where it biotinylates proteins in a proximity-dependent manner. The biotinylated proteins can then be affinity purified and identified by mass spectrometry. The original BioID uses a promiscuous BPL from E.coli (BirA R118G), however due to its relatively large size it occasionally hindered proper targeting of the proteins it was fused to. The second generation of the BioID method is based on a BPL from hyper thermophilic bacterium Aquifex aeolicus (A. aeolicus) that was mutated within the conserved biotin binding site (R40G) causing loss of BPL single substrate specificity. The promiscuous A. aeolicus BPL R40G referred to as BioID2 is the smallest known BPL. The smaller BioID2 not only improved targeting of the bait but also proved to be more efficient in biotinylating proximate proteins. The SS QD1 monoclonal antibodies were generated to support the recent developments in the BioID, a method that can be used to detect potential interacting proteins.
Conjugation: Unconjugated
Isotype: IgG2 kappa
Molecular weight: 27 kDa
Immunogen: GST fused to A. aeolicus BPL R40G (BioID2)
Immunogen Uniprot ID: O66837
Myeloma used: Sp2/0-Ag14
Target background: Monoclonal antibody used in the BioID method to detect protein-protein interactions. Background and Research Application BioID2 is a second-generation biotin protein ligase (BPL) that can be used to identify protein-protein interactions. In the BioID method a promiscuous biotin protein ligase (BPL) is fused to a protein of interests and expressed in vivo where it biotinylates proteins in a proximity-dependent manner. The biotinylated proteins can then be affinity purified and identified by mass spectrometry. The original BioID uses a promiscuous BPL from E.coli (BirA R118G), however due to its relatively large size it occasionally hindered proper targeting of the proteins it was fused to. The second generation of the BioID method is based on a BPL from hyper thermophilic bacterium Aquifex aeolicus (A. aeolicus) that was mutated within the conserved biotin binding site (R40G) causing loss of BPL single substrate specificity. The promiscuous A. aeolicus BPL R40G referred to as BioID2 is the smallest known BPL. The smaller BioID2 not only improved targeting of the bait but also proved to be more efficient in biotinylating proximate proteins. The SS QD1 monoclonal antibodies were generated to support the recent developments in the BioID, a method that can be used to detect potential interacting proteins.
Format: Liquid
Concentration: 1 mg/ml
Storage buffer: PBS with 0.02% azide
Storage conditions: -15° C to -25° C
Shipping conditions: Dry ice

Images

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Western blot analysis of SS QD1 monoclonal antibody. Total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line expressing BioID2 tagged to TorsinA ?E302/3 (6) without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS QD1 antibody. The predicted molecular weight of BioID2-TorsinA ?E302/3 fusion protein is 65 kDa.

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