SK-MEL-1 Cell Line

SK-MEL-1 are melanocytes established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras and were isolated from the skin of a 29-year-old, White male patient with melanoma.

Contributors

Inventor Institute
Herbert F. Oettgen Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161914
Cancer types: Skin cancer
Tissue: Skin
Gender: Male
Morphology: Spherical
Growth properties: Clusters in suspension
Cancer Types In Detail: Melanoma
Primary citation: Oettgen et al. 1968. Journal of the National Cancer Institute. 41: 827-843. PMID: 4879578.
Product description: SK-MEL-1 are melanocytes established from patient-derived tumour samples. This cell line expresses mutant B-Raf (V600E) and wildtype N-Ras and were isolated from the skin of a 29-year-old, White male patient with melanoma.
Gender: Male
Products or characteristics of interest: Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Shipping conditions: Dry Ice
Growth medium: EMEM suplemented with 10% FBS.
Subculture routine: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 105 cells/mL and maintain at 2 x 105 cells/mL. Medium Renewal: 2 to 3 times per week
Temperature: 37° C
Storage medium: Complete growth medium, 95%; DMSO, 5%
Recommended controls: –
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.

Fogh et al. 1977. Journal of the National Cancer Institute. 59: 221-226. PMID: 327080.

Oettgen et al. 1968. Journal of the National Cancer Institute. 41: 827-843. PMID: 4879578.

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