Piebaldism Melanocyte Cell Line

PiebaldismÂ?‚ is a rareÂ?‚ autosomal dominantÂ?‚ disorder ofÂ?‚ melanocyteÂ?‚ development. Common characteristics include a congenital whiteÂ?‚ forelock, scattered normal pigmented and hypo pigmentedÂ?‚ maculesÂ?‚ and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung’s disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool […]

Contributors

Inventor
Pranab K Das
Cat. #: 154104
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Immunology
Organism: Human
Model: Primary line
Product description: PiebaldismÂ?‚ is a rareÂ?‚ autosomal dominantÂ?‚ disorder ofÂ?‚ melanocyteÂ?‚ development. Common characteristics include a congenital whiteÂ?‚ forelock, scattered normal pigmented and hypo pigmentedÂ?‚ maculesÂ?‚ and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
Conditional: No
Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10Â?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100Â?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Parental cell line: Lesional skin of piebaldism patient
disease: Piebaldism
Format: Frozen
Shipping conditions: Dry ice
Growth medium: HAMs F10 supplemented with 10 ?„?žg/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100?„?žg/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)

Related Tools for Cell lines

Cat. # Tool Name
151425 TR146 Cell Line Key Info View Tool
151445 1-7HB2 Cell Line Key Info View Tool
151449 JIM1 Cell Line Key Info View Tool
151463 Omega-E Packaging Cell Line Key Info View Tool
151448 CMT 167 Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.