PiebaldismÄÂ? is a rareÄÂ? autosomal dominantÄÂ? disorder ofÄÂ? melanocyteÄÂ? development. Common characteristics include a congenital whiteÄÂ? forelock, scattered normal pigmented and hypo pigmentedÄÂ? maculesÄÂ? and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung’s disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
Gender:
Male
CRISPR:
Yes
Conditional:
No
Production details:
Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
Parental cell line:
Lesional skin of piebaldism patient
Disease:
Piebaldism;Piebaldism
Cat. #:
154104
Tool sub type:
10XUAS IVS p10
Unit size:
1×10^6 cells / vial
Organism:
Human
Gender:
Male
Model:
Primary line
Format:
Frozen
Shipping conditions:
Dry ice
Growth medium:
HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
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